Three‐month outcomes of aortic valve reconstruction using collagenous membranes (biosheets) produced by in‐body tissue architecture in a goat model: a preliminary study

Abstract Background Autologous pericardium is widely used as a plastic material in intracardiac structures, the pulmonary artery, and aortic valve leaflets. For reconstruction (AVRec) using Ozaki procedure, it has produced excellent clinical results over 10-year period. In-body tissue architecture (iBTA), which based on phenomenon of encapsulation foreign materials, can be to prepare autologous prosthetic tissues. In this preliminary study, we examined whether biosheets leaflet for glutaraldehyde-free AVRec by subchronic implantation experiments goats evaluated its performance compared with glutaraldehyde-treated AVRec. Methods Biosheets were prepared embedding molds two months into dorsal subcutaneous spaces goats. Autogenic (n = 4) cut shape then implanted annulus four three without glutaraldehyde treatment. was control. Valve function observed echocardiography. Results All survived three-month study With biosheets, surfaces very smooth and, histology, partially covered thin neointima (including endothelial cells). more thoroughly assimilated root pericardium. Conclusions first time, large animal could leaflets position may have ability assimilate native valves.